AGAR AGAR FOR BACTERIOLOGICAL USE

Inhibition of PCR by agar from bacteriological transport media.

We observed inhibition of PCR in throat swabs submitted in routine bacteriological transport media. Experimental studies showed that agar, which was extracted with DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent. No inhibitory effect was observed with a transport medium which did not contain agar.

GELRITE as an Agar Substitute in Bacteriological Media.

GELRITE gellan gum (formerly known as PS-60 and S-60) is a new naturally derived, highly purified polysaccharide which displays several interesting properties, including selfgelling. The suitability of GELRITE as an agar substitute was tested by evaluating the performance of several media selected from among those most commonly used in the isolation, identification, and enumeration of microorga...

The Introduction of Agar-agar into Bacteriology.

Among fundamental developments in bacteriology the elaboration of solid culture substrates will always hold first place. With the introduction of a method for isolating micro6rganisms and growing them in pure culture, bacteriologists possessed the means for studying what Ferdinand Cohn (1872) called "Die kleinsten lebenden Wesen." Credit for the discovery of the solid medium technique is common...

Blood agar, chocolate agar, and Mycobacterium tuberculosis.

We have read with interest the recent paper by Drancourt et al. in which they reviewed the use of blood agar medium for the primary isolation of Mycobacterium tuberculosis (2). They state that to their knowledge “no comparative study comparing the efficacy of blood-based agars and egg-based agars has been carried out, and even the ability of blood agar to support growth of M. tuberculosis was f...

Modification of the agar sausage of Ten Cate for bacteriological control.